Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5673011 | Journal of Virological Methods | 2017 | 9 Pages |
â¢Development of molecular diagnostic tools for infectious bronchitis virus (IBV).â¢Segregation of the IBV Egyptian variant II into two phylogroups detected.â¢Frequent co-infections with IBV, avian influenza and Newcastle disease observed.
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.