Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5674104 | Parasitology International | 2017 | 4 Pages |
â¢Sda-sialomucins increase in mouse intestinal mucosa after N. brasiliensis infection.â¢The increase in Sda-sialomucins was suppressed in IL-4Rαâ/â and SCID mice.â¢The expression of Sda-glycans was abrogated in IL-4Rαâ/â and SCID mice.â¢Administration of IL-13 led to expression of Sda-glycan in infected SCID mice.â¢IL-13/IL-4R axis is important for Sda-sialomucin production during infection.
Mucin is a major component of mucus in gastrointestinal mucosa. Increase of specific sialomucins having Sda blood group antigen, NeuAcα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ-, is considered to be associated with expulsion of the parasitic intestinal nematode Nippostrongylus brasiliensis. In this study, we examined the relationship between interleukin (IL)-13 pathway and expression of Sda-sialomucins in small intestinal mucosa with N. brasiliensis infection. Nematode infection induced marked increases in small intestinal mucins that reacted with anti-Sda antibody in wild type (wt) mice. However, this increase due to infection was supressed in IL-4 receptor α deficient (IL-4Rαâ/â) mice, which lack both IL-4 and IL-13 signaling via IL-4R, and severe combined immunodeficient (SCID) mice, which have defects in B- and T-lymphocytes. Analysis using tandem mass spectroscopy showed that Sda-glycans were not expressed in small intestinal mucins in IL-4Rαâ/â and SCID mice after infection despite the appearance of Sda-glycans in the infected wt mice. Inoculation of recombinant IL-13 into the infected SCID mice restored expression of Sda-glycan. Our results suggest that the IL-13/IL-4R axis is important for the production of Sda-sialomucins in the host intestinal mucosa with parasitic nematode infection.
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