Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5674979 | Virology | 2017 | 6 Pages |
Abstract
Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutralized new variant genotype 2 PEDV strains (LNCT2, LNSY, and Hjms), but it did not neutralize a genotype 1 PEDV strain (CV777), in vitro. Results showed that the epitope recognized by the 1B9 mAb lies in the spike protein, and that it is a conformational epitope. These findings confirm that allelic differences in the PEDV S gene between the G1 and G2 genotype strains led to changes in the S protein and, thus, differences in its immunogenicity.
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Immunology and Microbiology
Virology
Authors
Jianbo Liu, Hongyan Shi, Jianfei Chen, Xin Zhang, Zhaoyang Ji, Jing Yuan, Da Shi, Liyan Cao, Xiangdong Zhu, Hui Dong, Xiaobo Wang, Jialin Zhang, Li Feng,