Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5674989 | Virology | 2017 | 11 Pages |
Abstract
Although several studies have investigated the functions of influenza PA-X, the impact of different expressions of PA-X protein including full-length, truncated or PA-X deficient forms on virus replication, pathogenicity and host response remains unclear. Herein, we generated two mutated viruses expressing a full-length or deficient PA-X protein based on the A/California/04/2009 (H1N1) virus that expresses a truncated PA-X to understand three different expressions of PA-X protein on virus replication, pathogenicity and host immune responses. The results showed that expression of either full-length or truncated PA-X protein enhanced viral replication and pathogenicity as well as reduced host innate immune response in mice by host shutoff activity when compared to the virus expressing the deficient PA-X form. Furthermore, the full-length PA-X expression exhibited a greater effect on virus pathogenicity than the truncated PA-X form. Our results provide novel insights of PA-X on viral replication, pathogenicity and host immune responses.
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Authors
Jinhwa Lee, Hai Yu, Yonghai Li, Jingjiao Ma, Yuekun Lang, Michael Duff, Jamie Henningson, Qinfang Liu, Yuhao Li, Abdou Nagy, Bhupinder Bawa, Zejun Li, Guangzhi Tong, Juergen A. Richt, Wenjun Ma,