Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5675061 | Virology | 2017 | 7 Pages |
â¢Pathological characterization of FAdV-11 MX95 showed a low pathogenicity strain.â¢FAdV-11 MX95 is persistent in several organs of inoculated chickens.â¢Genome sequence of FAdV95 showed minimal genomic differences with high a pathogenicity strain.
In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1Ã106 TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326Â bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported.
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