Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5675266 | Virus Research | 2017 | 52 Pages |
Abstract
Using TB40/E/Cre, we: i) found genome length-associated differences in growth and ii) demonstrated its utility as a system capable of efficient transduction of exogenous proteins and regulation of their accumulation over periods as short as 2Â h. TB40/E/Cre is a powerful tool of broad applicability that can be adapted to study HCMV replication and cell biology in a variety of contexts.
Keywords
E. coliECFPMIEIFAHCMVHFFMIEPhpiORFPACDMEMFBSCPESV40 promoterTBCBACMOIDulbecco’s modified Eagle mediumdeionized watercytopathic effectsEscherichia colienhanced cyan fluorescent proteinstandard error of the meanlateEarlyCAMhours post infectionfetal bovine serumHuman cytomegalovirusimmediate earlyhuman foreskin fibroblastopen reading frameLuria brothSEMKanlinker regionmultiplicity of infectionKanamycinbacterial artificial chromosomeChloramphenicolunique short
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Authors
William L. Close, Amit Bhandari, Marwa Hojeij, Philip E. Pellett,