Generation of a novel human cytomegalovirus bacterial artificial chromosome tailored for transduction of exogenous sequences

Article ID	Journal	Published Year	Pages	File Type
5675266	Virus Research	2017	52 Pages	PDF

Abstract

Using TB40/E/Cre, we: i) found genome length-associated differences in growth and ii) demonstrated its utility as a system capable of efficient transduction of exogenous proteins and regulation of their accumulation over periods as short as 2Â h. TB40/E/Cre is a powerful tool of broad applicability that can be adapted to study HCMV replication and cell biology in a variety of contexts.

Keywords

E. coli ECFP MIE IFA HCMV HFF MIEP hpi ORF PAC DMEM FBS CPE SV40 promoter TBC BAC MOI Dulbecco’s modified Eagle medium deionized water cytopathic effects Escherichia coli enhanced cyan fluorescent protein standard error of the mean late Early CAM hours post infection fetal bovine serum Human cytomegalovirus immediate early human foreskin fibroblast open reading frame Luria broth SEM Kan linker region multiplicity of infection Kanamycin bacterial artificial chromosome Chloramphenicol unique short