Enzootic bovine leukosis in a two-month-old calf

•Enzootic bovine leucosis occurred even in two months old calf.•Inverse PCR is an effective method for evaluating the clonal growth of an enzootic bovine leucosis tumor cell.•The virus infection time may be important factors in the pathogenesis of enzootic bovine leucosis at an early age.

A two-month-old calf was diagnosed with leukosis on the basis of the clinical sign of enlarged, superficial lymph nodes. Serological and genetic tests for bovine leukemia virus (BLV) were performed because the calf was born from a cow infected with BLV. The serum had a weakly positive BLV antibody, and the BLV provirus was detected within neoplastic cells on performing polymerase chain reaction (PCR). Analysis of the BLV provirus integration site using inverse PCR revealed that the BLV integration site location was identical on all chromosomes in all tumor tissues examined. Thus, the tumor cells monoclonally proliferated following BLV infection. The present study shows that enzootic bovine leukosis can occur in a young animal, as in the two-month-old calf in our study.