Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5675559 | Virus Research | 2017 | 7 Pages |
Abstract
In this study, we described a novel and effective flower-like ZnO nanostructure assisted Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) method to detect Japanese Encephalitis Virus (JEV). The effects of different concentrations of ZnO nanoflower on the RT-LAMP reaction were investigated. With the increase of concentration of ZnO nanoflower, RT-LAMP reaction obtained optimization, until the concentration exceeded 1.5 nM, RT-LAMP reaction was inhibited. Made 1 nM as optimum concentration of ZnO nanoflower, we found that optimum RT-LAMP reaction temperature and time were 60 °C and 30 min, respectively. The optimization might be connected with good adsorption to DNA and thermal conductivity of ZnO nanoflower, but mechanism of the RT-LAMP reaction affected by ZnO nanoflower needs to be explored further.
Keywords
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Virology
Authors
Yonghua Ma, Yan Lu, Guiquan Guan, Jianxun Luo, Qingli Niu, Junlong Liu, Hong Yin, Guangyuan Liu,