Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5696714 | Reproductive BioMedicine Online | 2017 | 7 Pages |
Abstract
When fertility is impaired by anticancer treatment, spermatogonial stem cell transplantation (SSCT) could be used as a fertility restoration technique later on in life. Previously, we have demonstrated that a testicular cell suspension could be injected into a human cadaver testis, however, leakage to the interstitium was observed. In this study, injection of mouse testicular cells at an injection height of 50âcm (hydrostatic pressure) or via an automated injection pump (1400âµl, 2600âµl and 3000âµl) was evaluated. Significant difference in the filled radioactive volume was reached between the group in which 1400âµl was injected with an infusion pump and the groups in which 2600âµl (P = 0.019) or 3000âµl (P = 0.010) was injected. In all experimental groups green fluorescent protein positive (GFP+) cells were observed in the seminiferous tubules. In conclusion, a lower injection height did not resolve the leakage of the injected cells to the interstitium. Using the infusion pump resulted in more efficient filling of the seminiferous tubules with lower interexperimental variability. Although leakage to the interstitium was still observed, with further optimisation, the use of an infusion pump for clinical application is advantageous.
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Authors
Katrien Faes, Tony Lahoutte, Anne Hoorens, Herman Tournaye, Ellen Goossens,