Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5737470 | Neuroscience | 2017 | 12 Pages |
Abstract
Extra-virgin olive oil (EVOO) has several health promoting effects. Evidence have shown that EVOO attenuates the pathology of amyloid-β (Aβ) and improves cognitive function in experimental animal models, suggesting it's potential to protect and reduce the risk of developing Alzheimer's disease (AD). Available studies have linked this beneficial effect to oleocanthal, one of the active components in EVOO. The effect of oleocanthal against AD pathology has been linked to its ability to attenuate Aβ and tau aggregation in vitro, and enhance Aβ clearance from the brains of wild-type and AD transgenic mice in vivo. However, the ability of oleocanthal to alter the toxic effect of Aβ on brain parenchymal cells is unknown. In the current study, we investigated oleocanthal effect on modulating Aβ oligomers (Aβo) pathological events in neurons and astrocytes. Our findings demonstrated oleocanthal prevented Aβo-induced synaptic proteins, SNAP-25 and PSD-95, down-regulation in neurons, and attenuated Aβo-induced inflammation, glutamine transporter (GLT1) and glucose transporter (GLUT1) down-regulation in astrocytes. Aβo-induced inflammation was characterized by interleukin-6 (IL-6) increase and glial fibrillary acidic protein (GFAP) upregulation that were reduced by oleocanthal. In conclusion, this study provides further evidence to support the protective effect of EVOO-derived phenolic secoiridoid oleocanthal against AD pathology.
Keywords
PBSsAPPαsAPPβGLUT1RIPAAPPABCA1OleocanthalEVOOACMAβGFAPIL-6BSADMSOGLT1Astrocytesbovine serum albuminamyloid-βinsulin-degrading enzymeApoeapolipoprotein ENeuroinflammationAβ oligomersinterleukin-6IDEAlzheimer’s diseaseGlutamate transporterGlucose transporterAβoDimethyl sulfoxideExtra-virgin olive oilBlood–brain barrierBBBradioimmunoprecipitation assayphosphate buffer salineNeuronsGlial fibrillary acidic proteinamyloid precursor protein
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Yazan S. Batarseh, Loqman A. Mohamed, Sweilem B. Al Rihani, Youssef M. Mousa, Abu Bakar Siddique, Khalid A. El Sayed, Amal Kaddoumi,