Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5737900 | Neuroscience | 2017 | 12 Pages |
Abstract
Glioma, one of the most common cancers in human, is classified to different grades according to the degrees of malignancy. Glioblastoma (GBM) is known to be the most malignant (Grade IV) whereas low-grade astrocytoma (LGA, Grade II) is relatively benign. The mechanism underlying the pathogenesis and progression of glioma malignancy remains unclear. Here we report a quantitative proteomic study to elucidate the differences between GBM and LGA using liquid chromatography and tandem mass spectrometry followed by label-free quantification. A total of 136 proteins were differentially expressed in GBM for at least five folds in comparison with LGA. Ontological analysis revealed a close correlation between GBM-associated proteins and RNA processing. Interaction network analysis indicated that the GBM-associated proteins in the RNA processing were linked to crucial signaling transduction modulators including epidermal growth factor receptor (EGFR), signal transducer and activator of transcription 1 (STAT1), and mitogen-activated protein kinase 1 (MAPK1), which were further connected to the proteins important for neuronal structural integrity, development and functions. Upregulation of 40S ribosomal protein S5 (RPS5), Ferritin Heavy chain (FTH1) and STAT1, and downregulation of tenascin R (TNR) were validated as representatives by immune assays. In summary, we revealed a panel of GBM-associated proteins and the important modulators centered at the RNA-processing network in glioma malignancy that may become novel biomarkers and help elucidate the underlying mechanism.
Keywords
GBMTNRFTH1STRINGRab3AMAPK1LGAEGFRNCLSNCAFGF1LAMC1Syt1cancer pathogenesisMALDI-TOFFDRSynaptotagmin IDTTLC-MS/MSStat1Low-grade astrocytomaSearch Tool for the Retrieval of Interacting GenesKEGG یا Kyoto Encyclopedia of Genes and Genomes dithiothreitolFerritin Heavy ChainWorld Health OrganizationMass spectrometryFibroblast growth factor 1signal transducer and activator of transcription 1Matrix-assisted laser desorption/ionization time of flight mass spectrometryLabel-free quantificationfalse discovery rateNeurocanNucleolinmitogen-activated protein kinase 1liquid chromatography tandem mass spectrometryhigh-pressure liquid chromatographyHPLCWHOGlioblastomaEpidermal growth factor receptorAgrin
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Authors
Baibin Bi, Feng Li, Jisheng Guo, Cuiling Li, Ruirui Jing, Xin Lv, Xinjun Chen, Fengqin Wang, Kazem M. Azadzoi, Lin Wang, Yuguang Liu, Jing-Hua Yang,