Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5738728 | Neuroscience Letters | 2017 | 35 Pages |
Abstract
Oxygen and glucose deprivation (OGD) elicits a rapid and irreversible depolarization with a latency of â¼5Â min in intracellular recordings of hippocampal CA1 neurons in rat slice preparations. In the present study, we examined the role of cathepsin L in the OGD-induced depolarization. OGD-induced depolarizations were irreversible as no recovery of membrane potential was observed. The membrane potential reached 0Â mV when oxygen and glucose were reintroduced immediately after the onset of the OGD-induced rapid depolarization. The OGD-induced depolarizations became reversible when the slice preparations were pre-incubated with cathepsin L inhibitors (types I and IV at 0.3-2Â nM and 0.3-30Â nM, respectively). Moreover, pre-incubation with these cathepsin inhibitors prevented the morphological changes, including swelling of the cell soma and fragmentation of dendrites, observed in control neurons after OGD. These findings suggest that the activation of cathepsin L contributes to the irreversible depolarization produced by OGD.
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Authors
Shogo Kikuta, Yoshinaka Murai, Eiichiro Tanaka,