Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5738900 | Neuroscience Letters | 2017 | 10 Pages |
Abstract
Galanin-like peptide (GALP) is a 60 amino acid neuropeptide originally discovered from porcine hypothalamus, and is involved in the regulation of food intake in mammals. Since its discovery, GALP and its receptors (GALR1 and GALR2) have been characterized in mammals, but no publications are available on GALP in fish and other non-mammals. The present study aimed to characterize brain and intestinal GALP and its receptors using immunohistochemistry in a teleost, the goldfish (Carassius auratus), and to study its effects on feeding behavior. Immunostaining of brain sections shows the presence of GALP- and GALR1- and GALR2-like immunoreactive cells in different encephalic areas, including the telencephalon, some hypothalamic nuclei, the optic tectum, the torus longitudinalis and the cerebellum. Signal for GALP was also observed in the fasciculus retroflexus. In the gut, GALP-and GALR1 and GALR2 immunoreactive cells were detected in the mucosa. Results from the feeding study demonstrate that intracerebroventricular administration of GALP (1Â ng/g bodyweight) increases goldfish food intake at 1Â h post-injection. These observations form the first report on the presence of GALP in the fish brain and gut, and also on its modulatory role on fish feeding behavior. GALP, as in mammals, appears to be a functional neuropeptide in goldfish.
Keywords
intracerebroventricularPFAMS-222DAPINPYCCKICVNRLNATFasciculus retroflexusCARTGALPPBSNPPVOTEC4′,6-diamidino-2-phenylindoletorus longitudinalisImmunohistochemistryIHCFeedingcocaine- and amphetamine-regulated transcriptSubmucosaOptic tectumArcFishPhosphate-buffered salinemucosaBrainanterior tuberal nucleusarcuate nucleusbody weightparaformaldehydegalanin-like peptidecholecystokininGutNeuropeptide Y
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Authors
Elsie Tachie Mensah, Ayelén Melisa Blanco, Andrew Donini, Suraj Unniappan,