| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5741174 | Experimental Parasitology | 2017 | 6 Pages |
â¢Cysticerci exposed to RCB20 presented a greater induction of glycolylis.â¢Cysticerci exposed to RCB20 presented a greater induction of the TCA cycle.â¢RCB20 induced the energetic pathways.â¢RCB20 presents a greater effect on the metabolism than ABZSO.â¢RCB20 is a promising substitute for ABZSO.
Human cysticercosis caused by Taenia crassiceps is unusual; however, it is an useful experimental model for cysticercosis studies. Benzimidazole derivatives are important antihelminthic drugs widely used against helminths. A novel compound 6-chloro-5-(1-naphthyloxy) -2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative less polar and more lipophilic. The aim of this study was to detect the effect of the RCB20 on the in vitro energetic metabolism of T. crassiceps cysticerci. For this, products of the metabolism both produced and secreted/excreted (S/E) by the parasite were detected through spectrophotometry and high performance liquid chromatography after exposure to 6.5 and 13 μM of RCB20 and albendazole sulfoxide (ABZSO). There was a gradual increase in the concentrations of glucose not uptaken by parasites exposed to both concentrations RCB20 and ABZSO. There was a higher concentration of all the organic acids related to the tricarboxilic acid cycle int the parasites exposed to RCB20. The structural differences between RCB20 and ABZSO result in different targets within the parasite and in a greater induction of the energetic pathways, such as the glycolysis and the TCA cycle. RCB20 is a good candidate as a substitute for anthelminthic benzimidazoles due to a differentiated site of action with similar outcome.
Graphical abstractTaenia crassiceps cysticerci in vitro exposed to different concentrations of RCB20 presented an increase in the TCA cycle, impaired glucose uptake, use of glycogen reserves.Download high-res image (110KB)Download full-size image
