Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5747488 | Ecotoxicology and Environmental Safety | 2017 | 11 Pages |
Abstract
The ameliorative effect of H2 on aluminum (Al)-induced stress remains poorly understood. We treated maize seedlings with Al and hydrogen-rich water (HRW) to determine the roles of H2 in the alleviation of Al toxicity. Our results demonstrated that Al stress triggered damage to the photosynthetic apparatus, plant growth inhibition, and reactive oxygen species (ROS) production, and boosted lipid peroxidation. However, the addition of HRW at 75% saturation markedly alleviated Al toxicity symptoms through the promotion of root elongation. These responses were related to the significantly increased activities of typical antioxidant enzymes (CAT, APX, SOD, and POD). In vivo imaging of plasma membrane integrity, lipid peroxidation, and the level of ROS provided further evidence that HRW could improve Al tolerance. Our results also indicate that 100% HRW mitigated Al toxicity less than 75% HRW. Moreover, different concentrations of HRW significantly improved photosynthesis and increased nutrient uptake. We conclude that exogenous H2 supplementation could enhance Al tolerance by reestablishing redox homeostasis and maintaining nutrient homeostasis.
Keywords
APXMaize seedlingsPIABSPEPCNBTChlPPFDCSOTBARSPSIIASCCATPODFv/FmROSHydrogen peroxideHydrogen-rich waterAscorbic acidAluminumAntioxidant enzymesEDTAethylene diamine tetraacetic acidnitro blue tetrazoliumANOVAone-way analysis of varianceHRWsuperoxide radicalCAMChlorophyllSODSuperoxide dismutaseintercellular CO2 concentrationphotosynthetic photon flux densityPhotosystem IIreaction centerthiobarbituric acid reactive substancestranspiration ratenet photosynthetic rateStomatal conductanceH2O2Peroxidaseascorbate peroxidaseCatalaseCalmodulinReactive oxygen species
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Authors
Xueqiang Zhao, Qiuhong Chen, Yanmei Wang, Zhenguo Shen, Wenbiao Shen, Xiaoming Xu,