Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5747812 | Ecotoxicology and Environmental Safety | 2018 | 6 Pages |
Abstract
The present study demonstrated that the dual DNA staining with SYBR-14 and propidium iodide was effective in assessing viability of spermatozoa in marine invertebrates and that spectrofluorimetric analysis can be successfully employed to evaluate the percentage of live and dead spermatozoa. The method develop herein is simple, accurate, rapid, sensitive, and cost-effective, so it could be a useful tool by which marine pollutants may be screened for spermiotoxicity.
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Authors
Alessandra Gallo, Raffaele Boni, Elisabetta Tosti,