Combined effect of temperature and ammonia on molecular response and survival of the freshwater crustacean Gammarus pulex

•Combined effects of ammonia and high temperatures on survival are investigated.•The interaction between ammonia and increasing temperatures is antagonistic.•Hsp70 is overexpressed at high temperatures, but not at high ammonia concentrations.•Cross-tolerance mechanisms between ammonia and thermal stress are discussed.

Freshwater ecosystems are experiencing mounting pressures from agriculture, urbanization, and climate change, which could drastically impair aquatic biodiversity. As nutrient inputs increase and temperatures rise, ammonia (NH3) concentration is likely to be associated with stressful temperatures. To investigate the interaction between NH3 and temperature on aquatic invertebrate survival, we performed a factorial experiment on the survival and molecular response of Gammarus pulex, with temperature (10, 15, 20, and 25 °C) and NH3 (0, 0.5, 1, 2, 3, and 4 mg NH3/L) treatments. We observed an unexpected antagonistic interaction between temperature and NH3 concentration, meaning survival in the 4 mg NH3/L treatment was higher at 25 °C than at the control temperature of 10 °C. A toxicokinetic-toxicodynamic (TK-TD) model was built to describe this antagonistic interaction. While the No Effect Concentration showed no significant variation across temperatures, the 50% lethal concentration at the end of the experiment increased from 2.7 (2.1-3.6) at 10 °C to 5.5 (3.5- 23.4) mg NH3/L at 25 °C. Based on qPCR data, we associated these survival patterns to variations in the expression of the hsp70 gene, a generic biomarker of stress. However, though there was a 14-fold increase in hsp70 mRNA expression for gammarids exposed to 25 °C compared to controls, NH3 concentration had no effect on hsp70 mRNA synthesis across temperatures. Our results demonstrate that the effects of combined environmental stressors, like temperature and NH3, may strongly differ from simple additive effects, and that stress response to temperature can actually increase resilience to nutrient pollution in some circumstances.

Graphical abstractDownload high-res image (234KB)Download full-size image