Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
575258 | Journal of Hazardous Materials | 2016 | 9 Pages |
Abstract
A bacterial peroxidase-mediated oxidizing process was developed for biodegrading total petroleum hydrocarbons (TPH) in a sequencing batch reactor (SBR). Almost complete biodegradation (>99%) of high TPH concentrations (4Â g/L) was attained in the bioreactor with a low amount (0.6Â mM) of H2O2 at a reaction time of 22Â h. A specific TPH biodegradation rate as high as 44.3Â mgTPH/gbiomassÃh was obtained with this process. The reaction times required for complete biodegradation of TPH concentrations of 1, 2, 3, and 4Â g/L were 21, 22, 28, and 30Â h, respectively. The catalytic activity of hydrocarbon catalyzing peroxidase was determined to be 1.48Â U/mL biomass. The biodegradation of TPH in seawater was similar to that in fresh media (no salt). A mixture of bacteria capable of peroxidase synthesis and hydrocarbon biodegradation including Pseudomonas spp. and Bacillus spp. were identified in the bioreactor. The GC/MS analysis of the effluent indicated that all classes of hydrocarbons could be well-degraded in the H2O2-induced SBR. Accordingly, the peroxidase-mediated process is a promising method for efficiently biodegrading concentrated TPH-laden saline wastewater.
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Authors
Sakine Shekoohiyan, Gholamreza Moussavi, Kazem Naddafi,