Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
575801 | Journal of Hazardous Materials | 2016 | 10 Pages |
Abstract
We examined the effects of Cd on growth, lipid peroxidation, reactive oxygen species (ROS) accumulation, antioxidant enzymatic activity, and lignin content in the roots of two varieties of Vicia sativa. Treatment with Cd decreased plant growth and increased ROS and lipid peroxidation levels to a greater extent in the Cd-sensitive variety ZM than in the Cd-tolerant variety L3. Most hydrogen peroxide (H2O2) and superoxide anion (O2â) were accumulated in the cell walls and extracellular spaces in response to Cd treatments. Chemical assays and experiments using inhibitors showed that larger increases in H2O2 and O2â production in ZM than in L3 were probably attributed to elevated Cd-induced nicotinamide adenine dinucleotide-peroxidase (NADH-POD) activity. Cd treatment increased the accumulation of lignin and the guaiacol peroxidase (GPOD) activities in the apoplast more significantly in ZM root than in L3. Howerver, root laccase activity was higher in L3 than in ZM. Thus Cd toxicity induced significant lignification in the roots of V. sativa, and increases in H2O2 accumulation and apoplastic GPOD activity were likely responsible for this effect.
Keywords
CATTBARSNBTdehydroascorbate reductaseDHARAPXGSSHGSHNADPHdehydroascorbateAWFCWRapoplastic washing fluidPODDPIDABSPODASAGPODVicia sativa L.O2−ROSnitroblue tetrazoliumHydrogen peroxideAscorbic acidsuperoxide anioniPodLignificationImidazoleDHADiphenyleneiodoniumSODSuperoxide dismutasePlasma membraneIMZthiobarbituric acid reactive substancesNADHnicotinamide adenine dinucleotide phosphateNAD, nicotinamide adenine dinucleotideH2O2Peroxidaseascorbate peroxidaseCatalaseCadmiumreduced glutathioneoxidized glutathioneglutathione reductaseguaiacol peroxidaseReactive oxygen species
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Health and Safety
Authors
Haiyun Rui, Chen Chen, Xingxing Zhang, Zhenguo Shen, Fenqin Zhang,