Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5788503 | Science Bulletin | 2017 | 34 Pages |
Abstract
Viral reservoirs of HIV-1 are a major obstacle for curing AIDS. The novel animal models that can be directly infected with HIV-1 will contribute to develop effective strategies for eradicating infections. Here, we inoculated 4 northern pig-tailed macaques (NPM) with the HIV-1 strain HIV-1NL4.3 and monitored the infection for approximately 3 years (150 weeks). The HIV-1-infected NPMs showed transient viremia for about 10 weeks after infection. However, cell-associated proviral DNA and viral RNA persisted in the peripheral blood and lymphoid organs for about 3 years. Moreover, replication-competent HIV-1 could be successfully recovered from peripheral blood mononuclear cells (PBMCs) during long-term infection. The numbers of resting CD4+ T cells in HIV-1 infected NPMs harboring proviruses fell within a range of 2- to 3-log10 per million cells, and these proviruses could be reactivated both ex vivo and in vivo in response to co-stimulation with the latency-reversing agents JQ1 and prostratin. Our results suggested that NPMs can be infected with HIV-1 and a long-term viral reservoir was formed in NPMs, which might serve as a potential model for HIV-1 reservoir research.
Keywords
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Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Wei Pang, Gao-Hong Zhang, Jin Jiang, Hong-Yi Zheng, Lin-Tao Zhang, Xiao-Liang Zhang, Jia-Hao Song, Ming-Xu Zhang, Jia-Wu Zhu, Ai-Hua Lei, Ren-Rong Tian, Xiao-Ming Liu, Liguo Zhang, Guangxia Gao, Lishan Su, Yong-Tang Zheng,