Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5788807 | Science Bulletin | 2017 | 19 Pages |
Abstract
We described a novel single-cell RNA-seq technique called MR-seq (measure a single-cell transcriptome repeatedly), which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single cells by measuring each single cell twice. We demonstrated that MR-seq gave sensitivity and reproducibility similar to the standard single-cell RNA-seq and increased the positive predicate value. Application of MR-seq to early mouse embryos identified hundreds of candidate intra-embryonic heterogeneous genes among mouse 2-, 4- and 8-cell stage embryos. MR-seq should be useful for detecting differentially expressed genes among a small number of cells.
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Authors
Lu Yang, Zhaochun Ma, Chen Cao, Yuhao Zhang, Xinglong Wu, Raymond Lee, Boqiang Hu, Lu Wen, Hao Ge, Yanyi Huang, Kaiqin Lao, Fuchou Tang,