Genetic analysis of the reproductive axis in fish using genome-editing nucleases

Reproduction in fish is controlled by the brain-pituitary-gonad reproductive axis. Although genes of the reproductive axis are conserved from fish to humans, their in vivo functions are less clear in fish. Mutant lines of the reproductive axis have been systematically investigated in zebrafish and medaka using recently developed genome-editing nucleases. Here, we review recent progress in the genetic analysis of the reproductive axis in fish as well as the opportunities and challenges of applying genome-editing nucleases in fisheries.