Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
579876 | Journal of Hazardous Materials | 2011 | 7 Pages |
Abstract
Biodegradation of phenylurea herbicide isoproturon was studied in soil microcosm bioaugmented with a novel bacterial strain JS-11 isolated from wheat rhizosphere. The molecular characterization based on 16SrDNA sequence homology confirmed its identity as Pseudomonas aeruginosa strain JS-11. The herbicide was completely degraded within 20 days at ambient temperature with the rate constant of 0.08 dayâ1, following the first-order rate kinetics. In stationary phase, at a cell density of 6.5Â ÃÂ 109Â CFUÂ mLâ1, the bacteria produced substantially increased amounts of indole acetic acid (IAA) in the presence of tryptophan as compared with the control. Also, the bacteria exhibited a time-dependent increase in the amount of tri-calcium phosphate solubilization in Pikovskaya's medium. Further screening of the strain JS-11 for auxiliary activities revealed its remarkable capability of producing the siderophores and hydrogen cyanide (HCN), besides antifungal activity against a common phytopathogen Fusarium oxysporum. Thus, the versatile P. aeruginosa strain JS-11 with innate potential for multifarious biological activities is envisaged as a super-bioinoculant for exploitation in the integrated bioremediation, plant growth and disease management (IBPDM) in contaminated agricultural soils.
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Authors
Sourabh Dwivedi, Braj Raj Singh, Abdulaziz A. Al-Khedhairy, Javed Musarrat,