Homeopathic remedy made from Toxoplasma gondii reduces the number of bradyzoites in mice infected by the protozoan

IntroductionThe search for an effective remedy, with no side effects and with a low cost, is important both to prevent or reduce the damage in toxoplasmosis and to decrease economic losses. This work aims to compare the effect of different dynamizations of Toxoplasma gondii remedy made from the cysts of the parasite.MethodsIn a blind, controlled, randomized assay, 56 Swiss male mice, 60 days old, were divided into groups: BIOT-TG7, BIOT-TG17, BIOT - TG30, BIOT-TG60, BIOT-TG100, BIOT-TG200, GCInf - infected control group pretreated with 7% grain alcohol and GCN - uninfected and untreated control group. Medicines were prepared according to Brazilian Homeopathic Pharmacopoeia, with infected mice's brain macerated (20 T. gondii cysts/100 μL), in the following dynamizations D7, D17, D30, D60, D100 and D200. Pretreatment: 3 consecutive days before inoculation. Infection: At 60 days old, with 20 cysts ME49 strain-T. gondii, orally. Fundoscopy, ocular tonometry, Brain cysts counting, estimated number of bradyzoites/cyst and serum TGF-β (ELISA) were performed on the 60th day post-infection. Statistical comparison: Kruskal-Wallis test, 5% significance level.ResultsSmaller number of cysts (BIOT-TG17 and BIOT-TG200) and a smaller number of bradyzoites/cysts (p < 0.05), in BIOT-TG7, BIOT-TG100 and BIOT-TG200 was observed. 80% of animals from BIOT-TG100 group showed no alterations in fundoscopy and 20% presented subretinal mild hemorrhage around the optic nerve. There was no difference between groups in ocular tonometry and levels of TGF-β.ConclusionHigh dynamizations showed better results in mice infected with T. gondii different from the low dynamizations that had unsatisfactory results.