Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5816555 | Phytomedicine | 2014 | 9 Pages |
Abstract
TLRs are a family of receptors that play a critical role in the pathogenesis of diabetic nephropathy. TGP have been shown to have anti-inflammatory and immuno-regulatory activities. However, the relation between TGP and TLRs on diabetic nephropathy remains unknown. In this study, we examined effects of TGP on immune regulatory TLR2 and 4 in the kidney from streptozotocin-induced diabetic rats. TGP decreased the levels of 24 h urinary albumin excretion rate significantly in diabetic rats. Western blot analysis showed that TGP significantly inhibited the expression of TLR2 and 4, MyD88, p-IRAK1, NF-κB p65, p-IRF3, TNF-α and IL-1β. Quantitative real-time PCR analysis showed that the significantly increased levels of TLR2 and 4, and MyD88mRNA in the kidneys of diabetic rats were significantly suppressed by TGP treatment. Macrophages infiltration were also markedly increased in the kidneys of the diabetic rats, but were significantly inhibited by TGP in a dose-dependent manner. These results suggest that TGP has protective effects on several pharmacological targets in the progress of diabetic nephropathy by selectively blocking TLRs activation in vivo.
Keywords
NF-κBCCL-2TGPNF-κB p65TLRSTZIL-1βeNOSMCP-1ICAM-1MYD88TGF-β1TRIFstreptozotocininflammationTIR-domain-containing adapter-inducing interferon-βInterleukin-1βTransforming growth factor-β1tumor necrosis factor-αToll-like receptorendothelial nitric oxide synthasemyeloid differentiation factor 88TNF-αintercellular adhesion molecule-1Diabetic nephropathyMonocyte chemotactic protein-1Total glucosides of paeonyToll-like receptors
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Authors
Xing-xin Xu, Xiang-Ming Qi, Wei Zhang, Chao-Qun Zhang, Xiao-Xu Wu, Yong-Gui Wu, Kun Wang, Ji-Jia Shen,