Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5816903 | Phytomedicine | 2012 | 8 Pages |
Abstract
The antidiabetic actions of Castanospermum australe Cunn., seed (CAS) extract were evaluated in Poloxamer-407 (PX-407) induced T2DM rats. The CAS extract (100 and 150 mg/kg body weight) was administered orally once a day for 5 weeks after the animals were confirmed diabetic. A significant increase in blood glucose, HbA1c and serum insulin levels were observed in T2DM rats in comparison to citrate control rats. Treatment with CAS extract in T2DM rats reduced the elevated levels of blood glucose, HbA1c and insulin with significant (p â¤Â 0.001) improvement in OGT. The CAS extract treatment also increased (p â¤Â 0.001) the KITT and prevented increase in HOMA-R level in T2DM rats. The DPP-IV inhibitory potential of CAS extract showed IC50 value of 13.96 μg/ml whilst the standard Diprotin A displayed the IC50 value of 1.543 μg/ml. Molecular docking of the three reported alkaloids from the seeds of C. australe showed comparable DPP-IV inhibition with berberine. Our data suggest that CAS extract (150 mg/kg body weight) normalizes hyperglycemia in T2DM rats with strong DPP-IV inhibitory potential. The molecular docking showed that among the three alkaloids of seed extract 7-Deoxy-6-epi-castanospermine is a potent DPP-IV inhibitor similar to berberine.
Keywords
GIPkittPoloxamer-407HOMA-RT2DMHbA1cOGTTDPP-IVCASFBGPNAIC50Oral glucose tolerance testhomeostasis model assessment of insulin resistanceFasting insulinGlucose toleranceType 2 diabetesType 2 diabetes mellitusdipeptidyl peptidase-IVRetention timeWorld Health OrganizationInsulin sensitivity indexfasting blood glucoseInsulin resistanceDPP-IV inhibitorthe half maximal inhibitory concentrationHyperglycemiaglycosylated haemoglobinWHOglucagon like peptide-1glucose-dependent insulinotropic polypeptide
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Authors
Sudhanshu Kumar Bharti, Supriya Krishnan, Amit Kumar, Kaushal Kishore Rajak, Krishna Murari, Binod Kumar Bharti, Ashok Kumar Gupta,