Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-ÎºB regulated HIF-1Î± and PGE2 synthesis

Article ID	Journal	Published Year	Pages	File Type
5816991	Phytomedicine	2011	10 Pages	PDF

Abstract

In conclusion, we demonstrated that icariin has an in vitro inhibitory effects on osteoclasts differentiation that can prevent inflammatory bone loss. Icariin inhibited LPS-induced osteoclastogenesis program by suppressing activation of the p38 and JNK pathway.

Keywords

p38 ACP COX-2 BMP-2 BM-MSCs RANKL BMD PGE2 TRAP NF-κB HIF-1α α-MEM IκBα OPG M-CSF HEF nuclear factor of kappa light polypeptide gene enhancer in B-cells Cbfa1/Runx2 LPS IL-6 FBS Jnk c-Jun N-terminal kinases DMSO ERK1/2 extracellular signal-regulated kinase1/2 MAPKs p38 MAPK α-minimum essential medium MTT assay Osteoprotegerin Osteoclast interleukin-6 Icariin Gene expression Bone mineral density ELISA Enzyme-linked immunosorbent assay tumor necrosis factor-α Dimethyl sulfoxide fetal bovine serum Bone marrow-derived mesenchymal stem cells hypoxia inducible factor-1α TNF-α lipopolysaccharide ICR mice Nitric oxide polymerase chain reaction PCR Bone morphogenetic protein-2 Prostaglandin E2 mitogen-activated protein kinases receptor activator of NF-κB ligand