Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5822006 | Antiviral Research | 2015 | 10 Pages |
Abstract
Coxsackievirus B3 (CVB3) infection can cause acute myocarditis and chronic myocarditis, leading to dilated cardiomyopathy (DCM) with no effective therapeutic strategy. Therefore, we investigated the potential of absent in melanoma 2 (AIM2) to enhance the therapeutic efficacy of DNA vaccine against CVB3-induced chronic myocarditis. Mice were infected with CVB3 and then intranasally immunized with chitosan-pcDNA3.1 (mock), chitosan-pAIM2 (CS-pAIM2), chitosan-pVP1 (CS-pVP1), or chitosan-pAIM2 plus chitosan-pVP1 (CS-pAIM2/CS-pVP1) at 7, 21, and 35Â d. Therapeutic efficacies of various vaccines were evaluated at day 56Â d. Compared with CS-pVP1 immunization, CS-pAIM2/CS-pVP1 co-immunization significantly increased survival rate, improved cardiac function, as well as decreased myocardial injury and fibrosis, this result indicated that CVB3-induced chronic myocarditis was alleviated. CVB3-specific T lymphocyte proliferation and cytotoxic T lymphocyte responses of the CS-pAIM2/CS-pVP1 co-immunization group were also increased. More interestingly, CS-pAIM2/CS-pVP1 co-immunization could facilitate CVB3-specific multifunctional CD8+ T cell induction in the intestinal mucosa, and this induction was closely correlated with myocardial scores, this result indicated that CS-pAIM2/CS-pVP1 vaccine exhibits therapeutic efficacy by enhancing multifunctional CD8+ T cells. This study may represent a novel therapy for CVB3-induced chronic myocarditis.
Keywords
Chronic myocarditisDCMLVEFTCID50PRRsCVB3Coxsackievirus B3AIM2CVFLVEDdLVFSTnITroponin IDNA vaccineabsent in melanoma 2Dilated cardiomyopathycollagen volume fractionleft ventricular ejection fractiontissue culture infective dose 50%left ventricular fractional shorteningChitosanpattern recognition receptors
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Authors
Dafei Chai, Yan Yue, Wei Xu, Chunsheng Dong, Sidong Xiong,