| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5822653 | Antiviral Research | 2012 | 10 Pages |
Abstract
⺠Wheat-germ cell-free protein system simplified the production of DENV NS3 and NS5. ⺠A robust assay for NS3-NS5 interaction was established by AlphaScreen technology. ⺠Protease and helicase domains of NS3 were required for the binding to NS5. ⺠Both domains of NS3 exhibited competitive actions to the NS3-NS5 interaction.
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Authors
Hirotaka Takahashi, Chikako Takahashi, Nicole J. Moreland, Young-Tae Chang, Tatsuya Sawasaki, Akihide Ryo, Subhash G. Vasudevan, Youichi Suzuki, Naoki Yamamoto,