| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5833883 | International Immunopharmacology | 2011 | 8 Pages |
Abstract
⺠Tryptophan (W) residue is an important conserved residue among GPCRs. ⺠We explore the W5.43 in CB2 receptor and mutants for its roles by mutation and computer modeling. ⺠Our studies show that the W5.43 plays a key role in ligand binding and controlling the active states of the CB2 receptor.
Keywords
LDSCB2GPCRCB1HEK293HBSSTMHBGHTR-FRETDTTCannabinoid receptor CB2cAMPG-protein coupled receptorsSite-directed mutagenesisCyclic adenosine monophosphateadenylyl cyclaseTime-resolved fluorescence resonance energy transferTryptophanCNSdithiothreitolhuman embryonic kidney 293 cell linecentral nervous systemLithium dodecyl sulfatetransmembrane helixHank's balanced salt solutionComputer modelingwild typebovine growth hormoneG protein-coupled receptor
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Authors
Yuxun Zhang, Zhaojun Xie, Lirong Wang, Brielle Schreiter, John S. Lazo, Jurg Gertsch, Xiang-Qun Xie,