Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5841360 | Life Sciences | 2016 | 24 Pages |
Abstract
Maternal overnutrition (e.g., high-fat (HF) diet) during pregnancy and lactation is believed to cause oxidative stress and increase the risk of metabolic syndrome in offspring. In the present study, we investigated the effects of both maternal and offspring HF diets on metabolic syndrome risk and oxidative stress profiles in mice. Dams of the C57BL/6J mouse strain were fed a HF or control (CO) diet during gestation and lactation. Offspring were weaned at 3Â weeks of age. The female offspring were sacrificed at weaning, while the males were maintained on the HF or CO diet until 11Â weeks of age. Tissue samples, including those from liver, were collected from offspring at 3 and 11Â weeks of age, and lipids, phosphatidylcholine hydroperoxide (PCOOH, an oxidative stress marker), and gene expression were evaluated. Accumulation of lipids, but not PCOOH, was found in the livers of 3-week-old offspring from dams fed the HF diet. When the offspring were maintained on a HF diet until 11Â weeks of age, marked accumulation of both liver lipids and PCOOH was observed. PCOOH manifestation was supported by the expression of genes such as Gpx4, encoding a PCOOH degrading enzyme. These results suggest that the combination of maternal and offspring overnutrition causes marked oxidative stress in offspring, which accelerates metabolic syndrome. The present findings in offspring from infancy to adulthood may be useful for better understanding the cause-and-effect relationships between oxidative stress and metabolic syndrome development.
Keywords
GCLCphosphatidylcholine hydroxideACO2PCOOHCL-HPLCGPx4CPT2GCLMacyl-CoA oxidase 2GPx1SOD2SOD1PPARαCATTBARSactin betatriglycerideChemiluminescence detectionOxidative stressMetabolic syndromesuperoxide dismutase 1Superoxide dismutase 2ActbphosphatidylcholinePhospholipidthiobarbituric acid reactive substancesPhosphatidylcholine hydroperoxidehigh-fatCatalasecarnitine palmitoyltransferase 2glutamate-cysteine ligase modifier subunittotal cholesterolglutamate-cysteine ligase catalytic subunitControlglutathione peroxidase 1glutathione peroxidase 4Peroxisome proliferator activated receptor α
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Authors
Junya Ito, Kiyotaka Nakagawa, Shunji Kato, Taiki Miyazawa, Fumiko Kimura, Teruo Miyazawa,