Article ID Journal Published Year Pages File Type
5846188 Toxicology and Applied Pharmacology 2014 11 Pages PDF
Abstract

•Celastrol suppressed HIV-1 Tat-induced expression of pro-inflammatory genes.•Celastrol inhibited HIV-1 Tat -induced activation of JNK MAPK.•Celastrol inhibited HIV-1 Tat-induced activation of both NF-κB and AP-1.•Celastrol inhibited HIV-1 Tat-induced inflammatory responses via HO-1 induction.

HIV-1 Tat causes extensive neuroinflammation that may progress to AIDS-related encephalitis and dementia. Celastrol possesses various biological activities such as anti-oxidant, anti-tumor, and anti-inflammatory activities. In this study, we investigated the modulatory effects of celastrol on HIV-1 Tat-induced inflammatory responses and the molecular mechanisms underlying its action in astrocytes. Pre-treatment of CRT-MG human astroglioma cells with celastrol significantly inhibited HIV-1 Tat-induced expression of ICAM-1/VCAM-1 and subsequent monocyte adhesiveness in CRT-MG cells. In addition, celastrol suppressed HIV-1 Tat-induced expression of pro-inflammatory chemokines, such as CXCL10, IL-8, and MCP-1. Celastrol decreased HIV-1 Tat-induced activation of JNK MAPK, AP-1, and NF-κB. Furthermore, celastrol induced mRNA and protein expression of HO-1 as well as Nrf2 activation. Blockage of HO-1 expression using siRNA reversed the inhibitory effect of celastrol on HIV-1 Tat-induced inflammatory responses. These results suggest that celastrol has regulatory effects on HIV-1 Tat-induced inflammatory responses by blocking the JNK MAPK-AP-1/NF-κB signaling pathways and inducing HO-1 expression in astrocytes.

Graphical abstractDownload high-res image (159KB)Download full-size image

Related Topics
Life Sciences Environmental Science Health, Toxicology and Mutagenesis
Authors
, , , , , , ,