Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5846188 | Toxicology and Applied Pharmacology | 2014 | 11 Pages |
â¢Celastrol suppressed HIV-1 Tat-induced expression of pro-inflammatory genes.â¢Celastrol inhibited HIV-1 Tat -induced activation of JNK MAPK.â¢Celastrol inhibited HIV-1 Tat-induced activation of both NF-κB and AP-1.â¢Celastrol inhibited HIV-1 Tat-induced inflammatory responses via HO-1 induction.
HIV-1 Tat causes extensive neuroinflammation that may progress to AIDS-related encephalitis and dementia. Celastrol possesses various biological activities such as anti-oxidant, anti-tumor, and anti-inflammatory activities. In this study, we investigated the modulatory effects of celastrol on HIV-1 Tat-induced inflammatory responses and the molecular mechanisms underlying its action in astrocytes. Pre-treatment of CRT-MG human astroglioma cells with celastrol significantly inhibited HIV-1 Tat-induced expression of ICAM-1/VCAM-1 and subsequent monocyte adhesiveness in CRT-MG cells. In addition, celastrol suppressed HIV-1 Tat-induced expression of pro-inflammatory chemokines, such as CXCL10, IL-8, and MCP-1. Celastrol decreased HIV-1 Tat-induced activation of JNK MAPK, AP-1, and NF-κB. Furthermore, celastrol induced mRNA and protein expression of HO-1 as well as Nrf2 activation. Blockage of HO-1 expression using siRNA reversed the inhibitory effect of celastrol on HIV-1 Tat-induced inflammatory responses. These results suggest that celastrol has regulatory effects on HIV-1 Tat-induced inflammatory responses by blocking the JNK MAPK-AP-1/NF-κB signaling pathways and inducing HO-1 expression in astrocytes.
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