Ligustrazine attenuates oxidative stress-induced activation of hepatic stellate cells by interrupting platelet-derived growth factor-Î² receptor-mediated ERK and p38 pathways

Article ID	Journal	Published Year	Pages	File Type
5846592	Toxicology and Applied Pharmacology	2012	10 Pages	PDF

Abstract

âº Ligustrazine inhibits oxidative stress-induced HSC activation. âº Ligustrazine reduces fibrotic marker genes in HSCs under oxidative stress. âº Disruption of PDGF-Î²R-mediated ERK and p38 pathways was involved. âº Ligustrazine could be a promising antifibrotic candidate.

Keywords

ECM p38 MTS PPARγ ERK CTGF GAPDH FBS Jnk DMEM RTK MMP NF-κB α-SMA HSC c-Jun N-terminal kinase Dulbecco's modified Eagle Medium MAPK ROS Hydrogen peroxide α-smooth muscle actin Oxidative stress TIMP fetal bovine serum Hepatic stellate cell Connective tissue growth factor nuclear factor-κB Ligustrazine Extracellular matrix matrix metalloproteinase Tissue inhibitor of metalloproteinase H2O2 Extracellular regulated protein kinase mitogen-activated protein kinase Propidium iodide Glyceraldehyde phosphate dehydrogenase Reactive oxygen species receptor tyrosine kinases Peroxisome proliferator-activated receptor-γ