Mitochondrial aquaporin-8 knockdown in human hepatoma HepG2 cells causes ROS-induced mitochondrial depolarization and loss of viability

Article ID	Journal	Published Year	Pages	File Type
5846754	Toxicology and Applied Pharmacology	2012	9 Pages	PDF

Abstract

âº Aquaporin-8 is expressed in mitochondria of human hepatoma HepG2 cells. âº Aquaporin-8 knockdown impairs mitochondrial H2O2 release and increases ROS. âº Aquaporin-8 knockdown causes ROS-induced mitochondrial depolarization and cell death. âº Mitochondrial permeability transition blockage prevents depolarization and cell death.

Keywords

3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide AQP8 HepG2 Aquaporin-8 TMRM Dcf AQP OTC DCFH-DA MPTP 2′,7′-dichlorodihydrofluorescein diacetate 2′,7′-dichlorofluorescein MTT short interfering RNA ROS siRNA Hydrogen peroxide aquaporin mitochondrial permeability transition pore imm CSA Ornithine transcarbamylase cyclosporin A tetramethylrhodamine methyl ester Mitochondria Reactive oxygen species SCR