Nitrative DNA damage induced by multi-walled carbon nanotube via endocytosis in human lung epithelial cells

Article ID	Journal	Published Year	Pages	File Type
5846802	Toxicology and Applied Pharmacology	2012	10 Pages	PDF

Abstract

âºMulti-walled carbon nanotube (MWCNT) caused DNA damage in A549 cells. âºMWCNT formed 8-nitroguanine, a DNA lesion associated with inflammatory response. âºMWCNT was internalized into cells via caveolin- and clathrin-mediated endocytosis. âº8-Nitroguanine formation and iNOS expression involved these types of endocytosis. âºInternalized MWCNT plays a key role in inflammatory response and DNA damage.

Keywords

iNOS 8-Nitroguanine 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester CM-H2DCFDA MbCD CytoD MDC RNS MWCNT CNT FBS DMEM NF-κB PBS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Dulbecco's modified Eagle's medium MTT inducible NO synthase NO2−O2−ONOO−ROS DNA damage Endocytosis Tem analysis of variance ANOVA Bay Carcinogenesis fetal bovine serum Superoxide cytochalasin D nuclear factor-κB methyl-β-cyclodextrin Phosphate-buffered saline Monodansylcadaverine Transmission electron microscopy light microscopy Carbon nanotube Nitrite Nitric oxide Peroxynitrite reactive nitrogen species Reactive species Reactive oxygen species