Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5846872 | Toxicology and Applied Pharmacology | 2011 | 8 Pages |
Abstract
⺠The β1 and β3 subunits were compared by chimeragenesis, mutagenesis and modulators. ⺠Low β1 NCA binding was rescued by replacing its transmembrane helices with those of β3. ⺠GABA at 3 µM stimulated heteromers α1β1 and α1β3 binding levels more than 2-fold. ⺠Mutation at 15' position in TM2 reduced GABA stimulation of NCA binding. ⺠The open-state conformation largely determines GABAA receptor sensitivity to NCAs.
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Authors
Ligong Chen, Ling Xue, Kathleen M. Giacomini, John E. Casida,