The neuroprotective action of pyrroloquinoline quinone against glutamate-induced apoptosis in hippocampal neurons is mediated through the activation of PI3K/Akt pathway

Article ID	Journal	Published Year	Pages	File Type
5846935	Toxicology and Applied Pharmacology	2011	11 Pages	PDF

Abstract

âºPQQ attenuated glutamate-induced cell apoptosis of cultured hippocampal neurons. âºPQQ inhibited glutamate-induced Ca2+ influx and caspase-3 activity. âºPQQ reduced glutamate-induced increase in ROS production. âºPQQ affected phosphorylation of Akt and JNK signalings after glutamate injury. âºPI3K/Akt was required for neuroprotection of PQQ by modulating Bcl-2/Bax ratio.

Keywords

CREB NMDA TFAM AMPA N-methyl-d-aspartic acid DTNB PQQ NRF ERK WST-8 MDA Pyrroloquinoline quinine Jnk 5,5′-Dithio-bis (2-nitrobenzoic acid)terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling CCK-8 PI3K nuclear respiratory factors AKT signaling c-Jun N-terminal protein kinase MAPK ROS TUNEL Apoptosis SOD Superoxide dismutase cell counting kit-8 mitochondrial transcription factor A phosphatidylinositol-3-kinase malondialdehyde Hippocampal neurons Nitric oxide cAMP response element binding protein mitogen activated protein kinase Propidium iodide Pyrroloquinoline quinone Kainite extracellular signal-regulated kinases glutamate Reactive oxygen species