Effects of anti-cocaine vaccine and viral gene transfer of cocaine hydrolase in mice on cocaine toxicity including motor strength and liver damage

► Large cocaine doses (∼100 mg/kg) cause severe muscle weakness and liver damage in mice. ► A modest dose of cocaine hydrolase (1 mg/kg) or anti-cocaine antibody (8 mg/kg) is not protective. ► Combined enzyme and antibody block the measured signs of myo- and hepatotoxicity. ► Very high levels of cocaine hydrolase after in vivo gene transfer are protective alone.