Discovery and biological characterization of 1-(1H-indol-3-yl)-9H-pyrido[3,4-b]indole as an aryl hydrocarbon receptor activator generated by photoactivation of tryptophan by sunlight

Article ID	Journal	Published Year	Pages	File Type
5848453	Chemico-Biological Interactions	2011	10 Pages	PDF

Abstract

âº Sunlight has an important role in generating bioactive molecules that can activate the AHR. âº Identification of 1-(1H-indol-3-yl)-9H-pyrido[3,4-b]indole (IPI) as a new AHR-inducing tryptophan (TRP) photoproduct. âº IPI induces AHR-mediated P450 activity (EROD) and suppresses glucose output. âº IPI, a prominent component of photoactivated TRP, induces EROD additively with FICZ or TCDD. âº IPI was detected in tissue culture medium in ordinary ambient light conditions.

Keywords

EROD 6-formylindolo[3,2-b]carbazole ESIMS CEH FICZ IPI TCDD TOF MRM RP-HPLC CyP ATRP AHR CID Trp 2,3,7,8-Tetrachlorodibenzo-p-dioxin LC/MS MS/MS Abbreviations Collision-induced dissociation Electrospray Ionization Mass Spectrometry Tryptophan Time-of-Flight Cytochrome P4501A Cytochrome P450 Mass spectrometry Tandem mass spectrometry multiple reaction monitoring Liquid chromatography/mass spectrometry reverse phase high pressure liquid chromatography glucose output aryl hydrocarbon receptor