Quercetin modulates Nrf2 and glutathione-related defenses in HepG2 cells: Involvement of p38

Article ID	Journal	Published Year	Pages	File Type
5848512	Chemico-Biological Interactions	2012	11 Pages	PDF

Abstract

âº Quercetin induces a time-dependent activation of p38. âº Quercetin transiently stimulates Nrf2 translocation to later induce its inhibition. âº Quercetin regulates GSH-related antioxidant/detoxifying enzymes. âº In quercetin-treated cells p38 contributes to stabilize Nrf2. âº In quercetin-treated cells p38 modulates Nrf2, GSH-related enzymes and cell fate.

Keywords

GSH p38 PMSF DAPI AP-1 PKC ERK GAPDH CHX GPX PARP NAC GST GCs Kelch-like ECH-associated protein-1 Nrf2 DTT MCB c-jun amino-terminal kinase FBS Jnk GRB2 PI3K 6-diamidino-2-phenylindole MAPK N-acetyl-cysteine ROS Glutathione-related enzymes Akt/PKB dithiothreitol fetal bovine serum cycloheximide antioxidant response element Keap-1 phosphatidylinositol-3-kinase phenylmethylsulfonyl fluoride Monochlorobimane ARE activator protein-1 protein kinase B Protein kinase C mitogen-activated protein kinase growth factor receptor-bound protein 2 Quercetin extracellular regulated kinase Glutathione glutathione S-transferase glutathione reductase glutathione peroxidase glyceraldehyde-3-phosphate dehydrogenase Reactive oxygen species