Research paperEffect of cysteine and humic acids on bioavailability of Ag from Ag nanoparticles to a freshwater snail

•Direct uptake of particulate AgNP was quantified by subtracting out Ag+ uptake.•Addition of 25 nmol/l cysteine reduced, but did not eliminate uptake of 25 nmol/l Ag as AgNO3, PVP AgNPs or PEG AgNPs.•Biodynamic modeling quantified dissolved Ag, nanoparticulate Ag and bioavailable cys-Ag polymer uptake in the presence of cysteine.•The concentration and the form-specific rate constant of uptake determined the contribution of each avenue of uptake.•Addition Suwannee River Humic Acid (SRHA) enhanced uptake of Ag from PVP AgNPs, in contrast to cysteine.

Metal-based engineered nanoparticles (NPs) will undergo transformations that will affect their bioavailability, toxicity and ecological risk when released to the environment, including interactions with dissolved organic material. The purpose of this paper is to determine how interactions with two different types of organic material affect the bioavailability of silver nanoparticles (AgNPs). Silver uptake rates by the pond snail Lymnaea stagnalis were determined after exposure to 25 nmol l− 1 of Ag as PVP AgNPs, PEG AgNPs or AgNO3, in the presence of either Suwannee River humic acid or cysteine, a high-affinity thiol-rich organic ligand. Total uptake rate of Ag from the two NPs was either increased or not strongly affected in the presence of 1-10 mg l− 1 humic acid. Humic substances contain relatively few strong ligands for Ag explaining their limited effects on Ag uptake rate. In contrast, Ag uptake rate was substantially reduced by cysteine. Three components of uptake from the AgNPs were quantified in the presence of cysteine using a biodynamic modeling approach: uptake of dissolved Ag released by the AgNPs, uptake of a polymer or large (> 3 kD) Ag-cysteine complex and uptake of the nanoparticle itself. Addition of 1:1 Ag:cysteine reduced concentrations of dissolved Ag, which contributed to, but did not fully explain the reductions in uptake. A bioavailable Ag-cysteine complex (> 3 kD) appeared to be the dominant avenue of uptake from both PVP AgNPs and PEG AgNPs in the presence of cysteine. Quantifying the different avenues of uptake sets the stage for studies to assess toxicity unique to NPs.

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