Neuroprotective effects of MAPK/ERK1/2 and calpain inhibitors on lactacystin-induced cell damage in primary cortical neurons

Article ID	Journal	Published Year	Pages	File Type
5855444	NeuroToxicology	2011	12 Pages	PDF

Abstract

âº MAPK/ERK1/2 and calpain inhibitors attenuated the lactacystin-induced cell death in primary cortical neurons. âº Protection mediated by MAPK/ERK1/2 and calpain inhibitors were connected with attenuation of lactacystin-evoked DNA fragmentation without affecting the caspase-3 activity. âº The protective effects of MAPK/ERK1/2, but not calpain inhibitors were connected with attenuation of AIF protein level in cytosolic and nuclear fraction.

Keywords

GAPDH U0126 GDNF N-acetyl-l-cysteine Calpains mTOR NAC MDL28170 Jnk c-Jun N-terminal kinase ERK1/2 MAPK MTT ROS Neuronal apoptosis AIF DIV day in vitro Ubiquitin-proteasome system apoptosis inducing factor glia-derived neurotrophic factor lactate dehydrogenase LDH Lactacystin mammalian target of rapamycin mitogen-activated protein kinase calpeptin extracellular signal-regulated kinase 1/2 glyceraldehyde-3-phosphate dehydrogenase Reactive oxygen species UPS