Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5856730 | Regulatory Toxicology and Pharmacology | 2015 | 12 Pages |
Abstract
The objective of the present work was to study acute and subacute (28-days repeated dose) oral toxicity effect of glycosides based standardized fenugreek seed extract (SFSE-G) in vivo. SFSE-G was prepared by resin-based chromatography and standardized to glycosides namely trigoneoside Ib (76%) and vicenin 1 (15%). The acute oral toxicity (AOT) and subacute toxicity studies were performed in Swiss albino mice (5 mice/sex/group) as per OECD 425 (up-and-down procedure) and OCED 407 guidelines respectively. Acute oral administration of 5000Â mg/kg of SFSE-G showed 40% mortality with no mortality in lower dosages. The subacute oral administration of SFSE-G did not show observational or toxicological effects on the body or organ weights, food consumption, ophthalmic effects, locomotor activity, hematology, blood biochemistry, urinalysis, or histopathology at dose 250Â mg/kg. However, SFSE-G (1000Â mg/kg) showed mortality and minor alterations to body weight, relative liver weights, hematology and blood chemistry parameters related to treatment but it was within normal laboratory ranges. In conclusion, SFSE-G showed median lethal dose (LD50) more than 4350Â mg/kg and no-observed adverse effect levels (NOAEL) of 250Â mg/kg for both sexes during AOT and sub-acute toxicity study, respectively.
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Amit D. Kandhare, Subhash L. Bodhankar, V. Mohan, Prasad A. Thakurdesai,