Interpreting the biological relevance of bioinformatic analyses with T-DNA sequence for protein allergenicity

► Regulatory guidelines to predict protein allergenicity were used with crop genes. ► Alternate DNA reading frames and BLASTX/FASTA algorithms were employed. ► There were false positive alignments between endogenous proteins and allergens. ► Bioinformatics should be carefully considered from an allergy safety perspective. ► These findings impact how transgenic T-DNA may be assessed for safety.