Putting the parts together: Combining in vitro methods to test for skin sensitizing potentials

Article ID	Journal	Published Year	Pages	File Type
5857303	Regulatory Toxicology and Pharmacology	2012	16 Pages	PDF

Abstract

âº Comparative testing of 54 chemicals in five in vitro tests: DPRA, KeratinoSensTM, LuSens, hCLAT, mMUSST. âº Evaluation of the predictivity of the OECD QSAR Toolbox Vers. 2.0. âº Development of an in vitro test strategy having an accuracy similar to or better than the LLNA.

Keywords

ECVAM h-CLAT OECD NADPH Nrf2 DNCB HEPES MFI FITC RLU LLNA ITS ECHA FBS SDS TNBS KeratinoSens™PBS DPRA 1-chloro-2,4-dinitrobenzene 2,4,6-Trinitrobenzene sulfonic acid 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid AUC DMSO RP HPLC MTT human cell line activation test Local lymph node assay Direct Peptide Reactivity Assay European Chemicals Agency Integrated testing strategy QSAR Skin sensitization In vitro Room temperature Dimethyl sulfoxide Quantitative structure-activity relationship REACH Alternative methods Organisation for Economic Cooperation and Development sodium dodecyl sulfate fetal bovine serum Dendritic cells Control cells antioxidant response element Keap-1 Phosphate buffered saline fluorescein isothiocyanate Prediction model European Centre for the Validation of Alternative Methods area under the curve mean fluorescence intensity nicotinamide adenine dinucleotide phosphate ARE Kelch-like ECH-associated protein 1 Propidium iodide Reverse phase high performance liquid chromatography