Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5857936 | Reproductive Toxicology | 2016 | 8 Pages |
Abstract
The effects of verbascoside (VB), added at nanomolar concentrations during in vitro maturation (IVM) of juvenile sheep oocytes, on in vitro embryo development and its mechanisms of action at the oocyte level were analyzed. Developmental rates, after IVM in the presence/absence of VB (1Â nM for 24Â h; 1Â nM for 2Â h; 10Â nM for 2Â h), were evaluated. The bioenergetic/oxidative status of oocytes matured after IVM in the presence/absence of 1Â nM VB for 24Â h was assessed by confocal analysis of mitochondria and reactive oxygen species (ROS), lipid peroxidation (LPO) assay, and quantitative PCR of bioenergy/redox-related genes. The addition of 1Â nM VB during 24Â h IVM significantly increased blastocyst formation and quality. Verbascoside reduced oocyte ROS and LPO and increased mitochondria/ROS colocalization while keeping mitochondria activity and gene expression unchanged. In conclusion, supplementation with nanomolar concentrations of VB during IVM, in the juvenile sheep model, promotes embryo development by protecting the oocyte against oxidative stress.
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Authors
Nicola Antonio Martino, Federica Ariu, Daniela Bebbere, Manuel Filioli Uranio, Adriana Chirico, Giuseppina Marzano, Anna Maria Sardanelli, Angela Cardinali, Fiorenza Minervini, Luisa Bogliolo, Maria Elena Dell'Aquila,