Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5858877 | Reproductive Toxicology | 2014 | 11 Pages |
Abstract
Eight-week-old male Kunming mice were administered either melamine (MA, 30, 140, or 700Â mg/kg/day), a melamine and cyanuric acid mixture (MC, each at 15, 70, or 350Â mg/kg/day), or vehicle (control) for 3 consecutive days. Testicular toxicity was evaluated on days 1 and 5 after the final exposure. The testicular and epididymal weights and serum testosterone level were significantly decreased in the highest MC group (350Â mg/kg/day). Histopathologically, both MA and MC caused obvious lesions in the testis and epididymis, with significant increases in sperm abnormalities. By TEM, the blood-testis barrier was damaged dose dependently. TUNEL staining showed that both MA and MC induced increases in germ cell apoptosis. The Sertoli cell vimentin was collapsed in the treated animals as detected by immunohistochemical staining and Western blotting. This study demonstrated that both MA and MC treatments could disrupt the blood-testis barrier and cause a clear testicular toxicity.
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Authors
Lingling Chang, Ruiping She, Longhuan Ma, Hua You, Fengjiao Hu, Tongtong Wang, Xiao Ding, Zhaojie Guo, Majid Hussain Soomro,