Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5859384 | Toxicology | 2013 | 12 Pages |
Abstract
Antidepressants are generally used for treatment of various mood and anxiety disorders. Several studies have shown the anti-tumor and cytotoxic activities of some antidepressants, but the underlying mechanisms were unclear. Maprotiline is a tetracyclic antidepressant and possesses a highly selective norepinephrine reuptake ability. We found that maprotiline decreased cell viability in a concentration- and time-dependent manner in Neuro-2a cells. Maprotiline induced apoptosis and increased caspase-3 activation. The activation of caspase-3 by maprotiline appears to depend on the activation of JNK and the inactivation of ERK. Maprotiline also induced [Ca2+]i increases which involved the mobilization of intracellular Ca2+ stored in the endoplasmic reticulum. Pretreatment with BAPTA/AM, a Ca2+ chelator, suppressed maprotiline-induced ERK phosphorylation, enhanced caspase-3 activation and increased maprotiline-induced apoptosis. In conclusion, maprotiline induced apoptosis in Neuro-2a cells through activation of JNK-associated caspase-3 pathways. Maprotiline also evoked an anti-apoptotic response that was both Ca2+- and ERK-dependent.
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Authors
Chung-Ren Jan, Jian-An Su, Chih-Chuan Teng, Meei-Ling Sheu, Paul-Yann Lin, Miao-Ching Chi, Chia-Hao Chang, Wayne C. Liao, Chun-Chi Kuo, Chiang-Ting Chou,