Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5859691 | Toxicology Letters | 2016 | 11 Pages |
Abstract
Di(2-ethylhexyl) phthalate (DEHP) is currently the most commonly used phthalate for the production of flexible polyvinyl chloride. Phthalates including DEHP have been labeled as potential endocrine disruptors. The effect on the development of the neocortex, however, is unknown. To evaluate the neurodevelopmental effects of prenatal DEHP exposure at 1 and 100Â mg/kg/day or 100 and 500Â mg/kg/day in fetal and newborn mice, we performed a detailed histologic analysis of the developing dorsal telencephalon and neocortex. The observation of fetuses exposed to DEHP revealed reductions of proliferation and neurogenesis (1 and 100Â mg/kg) and an increase in cell death (500Â mg/kg). In addition, the newborns prenatally exposed to DEHP showed an abnormal neuronal distribution and a decrease in neurons. These findings suggest that prenatal DEHP exposure induces neurodevelopmental toxicity associated with the neural stem cell niche and corticogenesis.
Keywords
DEHPCldUPLPIdUNOAELDAPIDorsal telencephalonTuj1PBSCERHR4′,6-diamidino-2-phenylindoleH&Emono-(2-ethylhexyl) phthalatebromo-deoxyuridineBrdUProliferationCNSdi(2-ethylhexyl) phthalateembryonic daypostnatal daycentral nervous systemcortical plateAnogenital distancePhosphate-buffered salineventricular zoneNeurogenesisneocortexHematoxylin and EosinNo observed adverse effect levelAGD
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Authors
Munekazu Komada, Yuuya Gendai, Nao Kagawa, Tetsuji Nagao,