Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5859755 | Toxicology Letters | 2016 | 20 Pages |
Abstract
Application of cisplatin (DDP) for treating lung cancer is restricted due to its toxicity and lung cancer's drug resistance. In this study, we examined the effect of Jinfukang (JFK), an effective herbal medicine against lung cancer, on DDP-induced cytotoxicity in lung cancer cells. Morphologically, we observed that JFK increases DDP-induced pro-apoptosis in A549 cells in a synergistic manner. Transcriptome profiling analysis indicated that the combination of JFK and DDP regulates genes involved in apoptosis-related signaling pathways. Moreover, we found that the combination of JFK and DDP produces synergistic pro-apoptosis effect in other lung cancer cell lines, such as NCI-H1975, NCI-H1650, and NCI-H2228. Particularly, we demonstrated that AIFM2 is activated by the combined treatment of JFK and DDP and partially mediates the synergistic pro-apoptosis effect. Collectively, this study not only offered the first evidence that JFK promotes DDP-induced cytotoxicity, and activation of AIFM2 enhances apoptosis of human lung cancer cells undergoing toxicological stress, but also provided a novel insight for improving cytotoxicity by combining JFK with DDP to treat lung cancer cells.
Keywords
DDPCCK8TCMRT-qPCRCAMsRNATranscriptomeApoptosisKEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and GenomesDAVIDLung cancerCytotoxicitycisplatinCell Counting Kit 8traditional Chinese medicineGene ontologyquantitative real-time PCRDatabase for Annotation Visualization and Integrated Discoverypropidium iodine
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Authors
Jun Lu, Jian Chen, Nianjun Xu, Jun Wu, Yani Kang, Tingting Shen, Hualei Kong, Chao Ma, Ming Cheng, Zhifeng Shao, Ling Xu, Xiaodong Zhao,